The suite of analytical instrumentation within the Chemical Characterisation Facility also includes two modern nuclear magnetic resonance (NMR) spectrometers, both fitted with autosamplers for sample queuing and optimal analysis turnaround.
The dual systems offer ample flexibility for the elucidation of compound structure with access to the 400 MHz spectrometer catering towards more routine NMR analysis while the higher resolution 500 MHz instrument is generally reserved for more specialised experiments and longer acquisitions. In addition, the availability of three different probes for the 500 MHz spectrometer allows for a wide range of measurements to be carried on a range of samples types ranging from liquid samples (liquid probe) to low concentration liquid samples (cryo-probe) and solid samples (solid state probe) for those difficult to dissolve analytes.
In addition to the classic 1D NMR (1H and 13C) and 2D NMR experiments (e.g. NOESY, COSY), access to expert technical staff can support your project with more sophisticated characterisation experiments based around other nuclei (e.g. 19F, 31P, 11B, 29Si, 15N) or pulse sequences (e.g. HSQC-TOCSY).
Example of expertise and analysis capabilities using NMR spectroscopy:
- Full NMR structural characterization and verification for small molecules
- Sample purity analysis (% w/w) via quantitative NMR (with water or impurity quantification)
- CP-MAS ssNMR analysis for investigating insoluble materials (e.g. resins or catalysts)
- Advanced customized NMR experiments (e.g. Kinetics Analysis, 3-D Protein NMR, DOSY, T1/T2 Relaxometry, Quantitative NOE, No-Deuterium NMR reaction monitoring, etc.)
NMR spectrometers available for access include the following:
- Bruker Avance III (400-MHz, liquid probe, lower resolution, routine analysis)
- Bruker Avance Neo (500-MHz, liquid, solid and cryo-probes; higher resolution, routine and specialised analysis)
